Required Template Amounts and Concentrations

Service	Amount of Template Required	Minimum Concentration
* per kilobasepair to be sequenced, or per kilobase to be sequenced ** permits re-runs
Single Primer Extensions (Plasmid)	0.5 µg / extension (minimum) 1.5 µg /extension (preferred)**	0.10 µg/µl
Single-Strand Contig (Plasmid)	3 µg / kilobase*	0.10 µg/µl
Publication Quality (Plasmid)	6 µg / kilobasepair*	0.10 µg/µl
Single Primer Extension (PCR Product)	50 ng / extension (minimum) 150 ng /extension (preferred)**	10 ng/µl
Single-Strand Contig (PCR Product)	0.3 µg / kilobase*	10 ng/µl
Publication Quality (PCR Product)	0.6 µg / kilobasepair*	10 ng/µl
RCA (Liquid Cultures)	100 µl grown culture in LB (more info)	n/a
RCA (Picked Colonies)	Picked colony into 10 µl of growth medium (more info)	n/a