Single Strand Contig Service
Our Single-Strand Contig Service is offered for Plasmids, PCR Products, Single-Strand templates like M13 clones, and larger constructs such as Lambda and BAC clones. While not guaranteed, the accuracy of single-strand sequence data typically exceeds 99%. Sequencing Reactions are performed; primers are designed and synthesized to allow us to "Primer Walk" the region of interest. Project turnaround is dependent upon the size of the region to be sequenced and pre-existing knowledge of the expected sequence. Sequencing is typically completed at a rate of about 1.5 Kb to 2.0 Kb per week.
Here is what is included in this service:
Analysis of sequencing reactions on ABI 3730xl DNA Analyzer platform
BigDye Terminator or BigDye Primer methodologies
Overlapping sequencing analyses performed on a single strand
Assembly of overlapping sequence data
Design and synthesis of all necessary sequencing primers using state-of-the-art primer design software
Full support of templates which prove to be difficult to sequence
Quantifying template concentration
An electronic copy of the finished sequence
A map showing location and coverage of sequence data and sequencing primer locations (optional)
All excess sequencing primers (optional)
Hard copies of the electropherograms, and finished sequence (optional)
Order Forms: (Excel) (PDF)
Click Here to See The Requirements | Click Here for Turn Around Time Information
Templates must be purified prior to submission. Exception: If you are also requesting our RCA Service, you needn't purify your templates.
Click Here for a detailed table of volume and concentration requirements.
The quality of sequencing results is highly dependent on the quality of the DNA. Good results have been obtained from both cesium chloride purified and Qiagen column purified plasmids.
Project turnaround is dependent upon the size of the region to be sequenced and pre-existing knowledge of the expected sequence. Sequencing is typically completed at a rate of about 1.5 Kb to 2.0 Kb per week.
We recommend using FedEx Priority to ensure the timely and safe arrival of your valuable samples.